Search results “Chromosome analysis bone marrow”
Acute Myelogenous Leukemia (AML) & Chronic Myelogenous Leukemia (CML)for USMLE
Handwritten video lecture on Acute Myelogenous Leukemia (AML) and Chronic Myelogenous Leukemia (CML) for the USMLE. Will discuss Pathophysiology, Signs and Symptoms, Treatment and Prognosis Pathophysiology of Myelogenous Leukemia The stem cells are found in the bone marrow which gives rise to myeloid stem cells which then give rise to myelobast and then the granulocytes (Eosinophils, Basophils, Neutrophils) In Acute Myelogenous Luekemia there is an increase in Myeloblasts and may even involve myeloid stem cell which will affect red blood cells and platelets. In Chronic Myelogenous Leukemia there is an increase in Tyrosine Kinase which increases the number of the granulocytes that are found. ACUTE MYELOGENOUS LUEKEMIA Arrests at precursor stage with more than 20percent blasts in Bone Marrow. Blasts accumulate in bone marrow and goes to peripheral tissue. Common to have decreased Red Blood Cells and Platelets as Bone marrow gets crowded. Changes in Growth Factor causes arrest of development or decreased apoptosis. Myelodysplastic syndrome is a minor change in stem cells that is not cancerous yet, but it commonly develops into cancer. CLASSIFICATION – Currently classification is adopted by WHO which is based on therapeutic targets. 1. AML wiith recurrent abnormalities a. 8 21 translocation, b. t(15 17) Promyelocytic Leukemia i. Auer rods (M3). ii. PML RaR translocation (may be treated with retinoic acid) 2. AML with Myelodysplastic Syndrome (Poor Prognosis) 3. AML that is therapy related – due to cytotoxic agents 4. AML, not specified – classified from M0 to M7 CLINICAL - Sudden onset also may have thrombocytopenia, anemia, bone main and may affect liver, spleen, lymph node. After treatment patient may experience tumor lysis syndrome (high K, High Uric acid, High Phosphate, Low Calcium) INVESTIGATIONS – Usually show normocytic anemia and thrombocytopenia. Blood smear shows blasts which are myeloperoxidase positive. Bone marrow aspiration will show hypercellular with more than 20 percent blasts. Cytogenetic analysis help with prognosis. TREATMENT – Induction consists of 7 days of IV cytarabine with 3 days of short acting anthracycline to kills as much of blasts as possible. Consolidation to mop up left over with high dose cytarabine. If remission favorable and young age then continue more cycles of cytarabine. If no resmission or comorbidities than perform stem cell transplant and investigation therapies. PROGNOSIS – depends on age. As age increases the prognosis is worse CHRONIC MYELOGENOUS LUEKEMIA PATHOGENESIS – Translocation between chromosome 9 (ABL1) and Chromosome 22 (BCL). ABL is responsible for production of tyrosine kinase which is tightly regulated. ABL transfers over to BCL on chromosome 22 known as ABL BCR fusion and the Philadelphia chromosome. This leads to constant production of tyrosine kinase. CLINICAL – has more insidious onset and found as incidental finding. Chronic phase is symptomatic but can be controlled with treatment. Accelerated phase there is an increase in the number of blasts and will be less responsive to treatment. Final stage is blast crisis is when it transforms to AML with extramedullary syndromes. INVESTIGATIONS – High level of leukocytosis that are LAP negative to rule out leukemoid reaction. Cytogenetic analysis is diagnostic. Flow cytometry identifies CD Markers present. TREATMENT – In chronic phase give imatinib meyslete which is a tyrosine kinase inhibitor, but this is not a cure and the disease is always there. Accelerated blast crisis then look for hematopoietic stem cell transplant which is curative. Interferon alpha and Busulfan can be used while waiting for donor.
Views: 15131 the study spot
bone marrow for micronucleus
isolation of femur from wister albino rats and removal of bone marrow cells for micronucleus assessment.
Cytogenetics. Human chromosomes. Karyotype.
A chromosome is an organized structure of DNA and protein that is found in cells. It is a single piece of coiled DNA containing many genes, regulatory elements and other nucleotide sequences. ... http://en.wikipedia.org/wiki/Chromosome http://en.wikipedia.org/wiki/Cytogenetics http://en.wikipedia.org/wiki/Karyotype
Views: 60406 microveda
4 hour FISH Workflow for FFPE, Blood, and Bone Marrow Specimens
ProPath Comparison between Agilent probes and DAKO Histology Kit Agilent provides a comprehensive menu of FISH probes for rapid identification of a wide range of chromosomal aberrations across the genome. SureFISH's unique design methodology for specific targets provides excellent resolution with high sensitivity and specificity: Unique Design Methodology Agilent's Comprehensive Menu of FISH Probes Provides: Coverage of the Most Relevant Targets Broad Menu: Hundreds of relevant probes Excellent Performance High resolution compared to BACs High sensitivity and specificity Probes designed to meet ACMG guidelines for clinical cytogenetics Flexibility Probes offered in the following colors: Green( ex: 495nm; em:517nm) Red( ex:547nm; em: 565nm) Blue ( ex: 429nm; em: 470nm) Unique in silico design methodology for targeting specific regions Pre-labeled probes to reduce labor time Compatible with established FISH procedures and filter sets Quality Control Probe localization to correct chromosome, demonstrated by hybridization images for every probe Integration with Agilent CytoGenomics Data Analysis Software Visualizing the probe of interest in Agilent CytoGenomics software Link from CytoGenomics software to the SureFISH website for streamlined order process Regulatory Compliant SureFish probes are Analyte Specific Reagents. Analytical and performance characteristics are not established. SureFish probes are produced in an ISO 13485-certified facility that is registered with the FDA as a medical device manufacturing establishment. http://www.genomics.agilent.com/surefishArticle.jsp?pageId=3004&_requestid=797254 http://www.genomics.agilent.com/surefish-home.jsp?&_requestid=797450 http://www.dako.com/dist/propath-her2-iqfish-whitepaper_29087.pdf http://www.dako.com/dist/index/products/featured-solutions/her2-iqfish-pharmdx.htm#tab3
Views: 412 Life Trade - Egypt
Bone Marrow Smear Preparation
Specimen collection technique for bone marrow samples by Craig E. Litz, M.D. Director, ProPath Hematopathology.
Views: 29741 ProPath Pathology
Fanconi Anemia : Symptoms, Diagnosis, Treatment | Know How to Diagnose Fanconi Anemia
====================================================== SUBSCRIBE TO MY CHANNEL PLEASE.... https://www.youtube.com/channel/UCWDyU5Z3SKdlZ0tL8WLsvRw?sub_confirmation=1 ===================================================== How to Diagnose Fanconi Anemia. How Is Fanconi Anemia Diagnosed? | How Is Fanconi Anemia Diagnosed? | Fanconi Anemia Workup: Approach Considerations, CBC Count | Fanconi Anemia: Causes, Symptoms and Diagnosis - Healthline | Fanconi Anemia - NORD (National Organization for Rare Disorders) | Diagnosis of Fanconi Anemia: Chromosomal Breakage Analysis | Diagnosis of Fanconi Anemia By Chromosome Breakage Tests | People also ask What are the signs and symptoms of Fanconi anemia? How do you get Fanconi anemia? Can you cure Fanconi anemia? What is the meaning of Fanconi anemia? Keywords: #fanconi anemia genetic testing #fanconi anemia pictures #treatment of fanconi anemia #chromosome breakage test #fanconi anemia causes #fanconi anemia definition #fanconi anemia pathophysiology #fanconi anemia life expectancy ------------------------------------------------------------------------------------------------------- THANKS FOR WATCHING OUR CHANNEL -------------------------------------------------------------------------------------------------------- -~-~~-~~~-~~-~- Please watch: "🍳🍳⛹Child's Anemia Know How to Deal with Your Child's Anemia" https://www.youtube.com/watch?v=7CVvsGLuXoE -~-~~-~~~-~~-~-
Views: 908 how to easily
Genetic explanation of how hemophilia inherited
Hemophilia is a rare bleeding disorder in which the blood doesn't clot normally. If you have hemophilia, you may bleed for a longer time than others after an injury. You also may bleed inside your body (internally), especially in your knees, ankles, and elbows. This bleeding can damage your organs and tissues and may be life threatening. Overview Hemophilia usually is inherited. "Inherited” means that the disorder is passed from parents to children through genes. People born with hemophilia have little or no clotting factor. Clotting factor is a protein needed for normal blood clotting. There are several types of clotting factors. These proteins work with platelets to help the blood clot. Platelets are small blood cell fragments that form in the bone marrow—a sponge-like tissue in the bones. Platelets play a major role in blood clotting. When blood vessels are injured, clotting factors help platelets stick together to plug cuts and breaks on the vessels and stop bleeding. The two main types of hemophilia are A and B. If you have hemophilia A, you're missing or have low levels of clotting factor VIII (8). About 8 out of 10 people who have hemophilia have type A. If you have hemophilia B, you're missing or have low levels of clotting factor IX (9). Rarely, hemophilia can be acquired. "Acquired” means you aren't born with the disorder, but you develop it during your lifetime. This can happen if your body forms antibodies (proteins) that attack the clotting factors in your bloodstream. The antibodies can prevent the clotting factors from working. This article focuses on inherited hemophilia. Outlook Hemophilia can be mild, moderate, or severe, depending on how much clotting factor is in your blood. About 7 out of 10 people who have hemophilia A have the severe form of the disorder. People who don't have hemophilia have a factor VIII activity of 100 percent. People who have severe hemophilia A have a factor VIII activity of less than 1 percent. Hemophilia usually occurs in males (with rare exceptions). About 1 in 5,000 males are born with hemophilia each year.
Harvesting thru Analysis The New Cytogenetics Workflow - ADS Biotec
Harvesting thru Analysis The New Cytogenetics Workflow
Views: 102 ADS Biotec Omaha
Isolating MNC (Mono-Nuclear Cells) from Mouse Bone Marrow
This is a continuing procedure from the video series Mouse Bone Marrow Harvesting performed by our production lab. For more videos on Peripheral blood/Spleen/Bone Marrow Harvesting from Rat and Mouse, please visit C&M LabPro Youtube Channel. About Our Company C&M LabPro offers animal cells products isolated from mouse, rat, non-human primate. With 18 years of animal cell isolation experiences, our products are offered in highest quality with competitive pricing for researchers in life science and biotechnology fields worldwide. For detailed information and inquiry regarding our product categories, such as PBMC (Peripheral Blood Mononuclear Cell), Spleen, Bone Marrow, Purified Cells, and Astrocytes isolated from Rat, Mouse, and Non-Human Primate, please visit us at www.cmlabpro.com.
Views: 1342 C&M LabPro
What Happens If Lymphoma Is In Bone Marrow?
What happens if lymphoma is in bone marrow? Youtubebone marrow biopsy. Lymphoma facts, types, symptoms, and treatment. If you have hodgkin's lymphoma that's spread through one or more organs outside of your lymphatic system, you'll be diagnosed with stage 4 the condition. The whole test takes 15 to 20 your guide symptoms of non hodgkin lymphoma. Follicular lymphoma may go away without treatment. Long term survival depends on the type of non hodgkin's lymphoma and stage disease when it is diagnosed. If lymphoma is found in your bone marrow, you might need another marrow biopsy the future to check response treatment. Hl can be diagnosed if typical r s cells or their mononuclear 16 oct 2010 bone marrow, found in the center of most large bones, is a spongy material that produces red blood cells, white and platelets. Not everyone with lymphoma needs a bone marrow biopsy specimens from 317 patients non hodgkin's (nhl) obtained at initial staging were evaluated for the presence of or benign lymphoid aggregates. Stem cell and bone marrow transplants. Some patients experience slight 4 apr 2004 review article. A bone marrow biopsy is used to evaluate how well the functioning and determine if lymphoma present. The doctor or nurse takes this needle out and puts in a second one if you are having trephine biopsy as well. Recurrent recurrent lymphoma is that has come back after treatment 1 sep 2017. The chemotherapy has a good chance of killing the cancer cells but also kills stem in your bone marrow. The bone marrow aspiration and biopsy are usually done at the same time. Googleusercontent search. Thirty two percent (102 patients) had lymphoma in their bone marrow, and 9. About lymphoma in the bone marrow patients against. Hodgkin lymphoma bone marrow test cancer research ukcancer uk. Htm "imx0m" url? Q webcache. What you should know about bone marrow biopsy for the staging transplant tests hodgkin lymphoma american cancer society. This can cause low blood cell counts, such as red if this happens, you may have similar symptoms to these. Some people have a sudden, sharp pain. You should see your doctor if you. About lymphoma in the bone marrow patients against lymphomation. For blood cells 15 nov 2016 hodgkin's lymphoma. With types of nhl that have spread to the bloodstream or bone marrow, it may be difficult obtain uncontaminated cells can used, even after treating them in a laboratory remove kill 28 mar 2017 marrow aspiration and biopsy these tests are not used diagnose hodgkin lymphoma, but they done diagnosis is made see if lymphoma. The aim is to get a small amount of marrow out in one piece. Lymph flows through the lymph nodes, as well other lymphatic tissues including spleen, tonsils, bone marrow, and thymus gland. The patient is closely watched for signs or symptoms that the disease has come back 22 nov 2017 in this article, learn about types of bone marrow cancer, including multiple myeloma, leukemia, and lymphomas. Bone marrow biopsy (bmb) is commonl
Views: 98 E Info
How did God create Adam (a.s.)? A look at Various Opinions
In this clip taken from his weekly Q&A, Mufti Abu Layth explores some of the opinions and theories on how Adam a.s. was created by Allah.
Engraftment Monitoring Following Human Stem Cell Transplantation
Chimerism is an extremely rare disorder that mixes the chromosomal population in a single organism. Chimerism may manifest as the presence of two sets of DNA, or organs that do not match the DNA of the rest of the organism. In some cases, hermaphroditic characteristics can be signs of chimerism. Contemporary use of the term chimerism derives from the idea of a mixed entity, referring to someone who has received a transplant of genetically different tissue. Engraftment monitoring, also called chimerism testing, by DNA utilizes methodology commonly used in human identity testing. Dr. Shalini Pereira talks about how this new testing technology identifies genetic profiles of a recipient and donor(s) after a stem cell transplant, and can be used to evaluate the extent of mixture in the recipient's peripheral blood, bone marrow or other tissue. To see more videos from the University of Washington visit uwtv.org.
Views: 2402 UWTV
The Great Search - HLA Typing, Donor Selection and Beyond
2015 Clinical Education Conference Lisa Getzendaner, PA-C, MHS, Fred Hutchinson Cancer Research Center
Views: 19947 ASBMT
Soft Tissue Found Inside a Dinosaur Bone!
In 2003, an exceptionally well preserved T. rex leg bone was unearthed in the Cretaceous rocks of Montana. Upon close examination, Dr. Mary Schweitzer found the bone to contain soft tissue inside - tiny blood vessels and proteins! Some claim that this is evidence of a young Earth, a recent extinction of the dinosaurs. Could this be, or is there another explanation? ======== Support us on Patreon: https://www.patreon.com/statedclearly Get a T. rex t-shirt: https://www.redbubble.com/people/statedclearly/works/28105774-gloating-t-rex?asc=t&p=t-shirt Visit Dr. Mary Schweitzer’s website: https://molecularpaleo.wordpress.ncsu.edu/ ======== Papers cited in this animation: ORIGINAL DISCOVERY OF SOFT TISSUE: Soft Tissue Vessels and Cellular Preservation in Tyrannosaurus rex: http://science.sciencemag.org/content/307/5717/1952 Soft tissue and cellular preservation in vertebrate skeletal elements from the Cretaceous to the present: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1685849/ CRITIQUE OF DISCOVERY: Dinosaurian Soft Tissues Interpreted as Bacterial Biofilms: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2483347/ DEFENSES OF DISCOVERY: Influence of Microbial Biofilms on the Preservation of Primary Soft Tissue in Fossil and Extant Archosaurs: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2953520/ Molecular analyses of dinosaur osteocytes support the presence of endogenous molecules: http://www.thebonejournal.com/article/S8756-3282(12)01318-X/fulltext Testing the Hypothesis of Biofilm as a Source for Soft Tissue and Cell-Like Structures Preserved in Dinosaur Bone: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4771714/ 10 million year old frog bones with marrow preserved: https://pubs.geoscienceworld.org/geology/article-abstract/34/8/641/129601/high-fidelity-organic-preservation-of-bone-marrow?redirectedFrom=fulltext IRON’S ROLE IN PRESERVATION: A role for iron and oxygen chemistry in preserving soft tissues, cells and molecules from deep time: http://rspb.royalsocietypublishing.org/content/281/1775/20132741 OTHER DINOSAURS WITH SOFT TISSUE DISCOVERED: Mass Spectrometry and Antibody-Based Characterization of Blood Vessels from Brachylophosaurus canadensis: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4768904/ Evidence of preserved collagen in an Early Jurassic sauropodomorph dinosaur revealed by synchrotron FTIR microspectroscopy: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5290320/ Soft sheets of fibrillar bone from a fossil of the supraorbital horn of the dinosaur Triceratops horridus: https://www.ncbi.nlm.nih.gov/pubmed/23414624 Hemoglobin-derived porphyrins preserved in a Middle Eocene blood-engorged mosquito: https://www.ncbi.nlm.nih.gov/pubmed/24127577 Molecular preservation in Late Cretaceous sauropod dinosaur eggshells https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1599869/
Views: 49716 Stated Clearly
Peripheral Blood
Peripheral blood cell examination and white cell identification. Created by Kristi Lew of the Division of Medical Laboratory Science, University of Alberta
Views: 12250 HematoReview
Andro Spin - Sperm Separation System by Sperm360
Andro Spin - a centrifuge machine designed to work at 37 degree Celsius, that is same as the in-vivo temperature, to maintain the natural functionality of sperm
Views: 10795 Sperm 360
Hanabi PVI Metaphase Spreader - ADS Biotec
The HANABI PVI Metaphase Spreader has been designed to provide optimized environmental conditions for metaphase chromosome spread preparations. Temperature, humidity and airflow are set and rapidly stabilized so when chromosomes are dropped and spread on microscope slides, they are dried in a consistent and reproducible manner. Excessive spreading and chromosome overlaps are minimized, giving rise to better yields of analysable metaphases. The chromosome spreading process for conventional G-banding (or equivalent) and FISH analysis of samples derived from blood cells or bone marrow samples, is now more streamlined and can easily be carried out regardless of skill level. Protocols requiring cells and chromosomes are spread on the entire slide can be achieved using the tilted slide. Please visit http://www.adsbiotec.com/product/hanabi-pvi-metaphase-spreader/ for more information.
Views: 332 ADS Biotec Omaha
Is PV Genetic?
The proposed algorithm is based on genetic algorithms (gas) and it can estimate the current (imp) voltage (vmp) at maximum power point by jak2 mutation a change found in about 9 out of 10 people with polycythemia vera (pv). Pv) mpn research foundation. Affected individuals may also have excess white blood cells and clotting cell fragments called platelets. As a result instead, most cases of polycythemia vera are associated with genetic changes (mutations) that somatic. Restriction fragment length polymorphism and virulence analyses were used to evaluate the population structure of xanthomonas oryzae pv. The total capacity of hpws is determined based on estimated annual power demand, average wind speed and sun radiation. The familial predisposition was consistent with autosomal dominant inheritance incomplete penetrance. Googleusercontent search. Two dna sequences from xoryzae, is1112, an insertion sequence, t h e o r y. The disease onset is similar in familial and sporadic cases (median age at diagnosis 60 years; In general juvenile pv are rare). This results in three pv92 genotypes (, or )Pv) mpn research foundation. Almost all patients with pv have a mutation of the jak2 (janus kinase 2) gene trigger for polycythemia vera (pv) and other myeloproliferative neoplasms (mpns) isn't known. However, researchers have discovered that pv and other mpns may be caused by non inherited genetic mutations affecting proteins work in signaling pathways cells 15 nov 2015a progressive disease develops slowly will likely get worse over time. Oryzae (xoo), was genetically dissected by using 2 mapping populations and 10 xoo races. The pv92 genetic system has only two alleles indicating the presence ( ) or absence of alu transposable element on each paired chromosomes. Pv is a rare blood condition. In some parts of schuylkill, carbon and luzerne counties, more people than expected have pv compared with other locations. In rare cases, the mutation to a gene that causes pv does occur in egg or sperm cells, which increases risk person with will pass on their children polycythemia vera (pv) is one of related group blood cancers known as myeloproliferative neoplasms (mpns) cells bone marrow produce do not develop and function normally. Optimal placement of hybrid pv wind systems using genetic algorithm. In this paper, a new genetic algorithm based approach was presented for the reconfiguration. These extra cells and platelets cause the blood to be thicker than normal. The system tries to maximize the obtained complete resistance (cr) and partial (pr) of rice (oryza sativa l. To its bacterial pathogen, xanthomonas oryzae pv. This experiment examines pv92, a human specific alu insertion on chromosome 16. Genetics and pv what does it all mean? . Each pv and wind unit is abstract the purpose of reconfiguration process in systems to find connection structure highest amount power obtained by system under partial shading conditions. Polycythemia vera genetics home referencegene
Chronic Myeloid Leukemia Understanding Your Diagnosis
Unending Myeloid Leukemia Understanding Your Diagnosis. In the event that you've been as of late determined to have ceaseless myeloid leukemia, you presumably have a considerable measure of inquiries. Get some answers concerning this kind of tumor, including how it begins and its causes. Endless myeloid leukemia is now and then called ceaseless myelogenous leukemia, or just CML. Leukemia is a sort of growth that starts in the blood-framing cells inside the bone marrow before spreading through the blood. Understanding Bone Marrow: Bone marrow is a delicate substance found inside the bones in the body. It's comprised of a few kinds of cells, including blood undeveloped cells. These cells form into every one of the cells that make up blood, for example, white platelets, red platelets, and platelets. Some white platelets are called lymphocytes, and work to battle disease. The other platelets, including staying white cells, red cells, and platelets, are called myeloids. Endless Myeloid Leukemia: What Is It?. CML is alluded to as "endless" on the grounds that it is a long haul issue in which the destructive cells develop over an expanded timeframe. The cells are permitted to halfway grow, however don't completely develop. The "myeloid" grouping originates from the kind of cells influenced. There are three periods of CML: interminable, quickened, and impact emergency. The vast majority are generally analyzed amid the principal stage when cells are developing gradually. Amid the quickened stage, the sick cells start developing all the more rapidly. On the off chance that left untreated, CML may advance into the impact emergency stage. This is the point at which the bone marrow falls flat and genuine draining and disease can create. Manifestations of CML. Manifestations have a tendency to grow step by step. They can shift contingent upon which period of CML you're in, yet frequently include: *fatigue, in all likelihood because of weakness. *rash. *bruising or draining effortlessly. *bone and joint agony. *enlarged spleen. *increased danger of contamination. How Can It Start?. CML gives off an impression of being for the most part hereditary. A great many people who are analyzed have an unusual chromosome called the Philadelphia chromosome that makes another quality. This quality advises the platelets to make excessively of the tyrosine kinase protein. This enables the infected platelets to replicate too rapidly and live too long. As these cells develop, they don't leave enough space for solid platelets. This procedure can take quite a while. Some of the time individuals don't know they have CML until the point that years after the malignancy cells started to create. What Are the Causes and Risk Factors?. Despite the fact that CML is typically connected with a quality transformation, take note of that it's not innate. It's trusted that the quality change occurs sooner or later after the individual is conceived. While it's as yet not clear precisely how or why this change happens, some hazard factors include: *male sexual orientation. *older age. *exposure to radiation, including a few kinds of tumor radiation treatment. A great many people with CML are treated with drugs called tyrosine kinase inhibitors, or TKIs. These are pills that objective the protein made by the Philadelphia chromosome. Now and again, oral chemotherapy drugs are likewise used to lessen the quantity of strange white platelets. These medications, alone or in blend, are frequently enough to put the leukemia into abatement. Different methodology can likewise be considered, including undifferentiated cell transplants or bone marrow transplants. All Photos Licensed Under CC Source : www.pexels.com www.pixabay.com www.commons.wikimedia.org
Views: 2187 Natural Step
Views: 140 Aizaz Chaudhary
Tissue Typing
Views: 6995 Brilynn Schrader
Understanding Bone Marrow Transplantation - from the Clinical and Personal Perspectives
A bone marrow transplant is a multi-step procedure to replace damaged or destroyed bone marrow with healthy bone marrow stem cells, and it is used to treat certain blood cancers like lymphoma and leukemia, as well as aplastic anemia and other immune and genetic diseases. This talk discussed current treatment regimens. It also addressed the complexities that can be overwhelming for the family, as well as the patient, of going through bone marrow transplantation. Speakers: David Miklos, MD Bonnie Willdorf, author of Dancing with Cancer – Maladies and Miracles in Stem Cell Transplantation
Introducing the HANABI Harvesters - ADS Biotec
The HANABI Metaphase Chromosome Harvesters are the first systems to fully automate the multi-step process of preparing and harvesting metaphase cells derived from blood and bone marrow cell cultures and from other cell suspension cultures for cytogenetic analyses. Centrifugation, aspiration, addition of hypotonic and fixative solutions, and cell resuspension are all automated. Walk away reliability and low maintenance significantly reduce FTE time associated with the manual harvesting process. Please visit http://www.adsbiotec.com/product/chromosome-harvesting/ for more information.
Views: 448 ADS Biotec Omaha
Scientists Discovered Message from God in Human DNA Code!!
Scientists Discovered Message from God in Human DNA Code! Here's What God Said,"Hello my children. This is Yahweh, the one true Lord. You have found creation's secret. Now share it peacefully with the world. ancient Aramaic Here's the source http://dailycurrant.com/2013/02/01/message-god-hidden-dna-sequence/
Engraftment Monitoring Following Human Stem Cell Transplantation - Shalini Pereira, Ph.D.
Chimerism is an extremely rare disorder that mixes the chromosomal population in a single organism. Chimerism may manifest as the presence of two sets of DNA, or organs that do not match the DNA of the rest of the organism. In some cases, hermaphroditic characteristics can be signs of chimerism. Contemporary use of the term chimerism derives from the idea of a "mixed" entity, referring to someone who has received a transplant of genetically different tissue. Engraftment monitoring, also called chimerism testing, by DNA utilizes methodology commonly used in human identity testing. Dr. Shalini Pereira talks about how this new testing technology identifies genetic profiles of a recipient and donor(s) after a stem cell transplant, and can be used to evaluate the extent of mixture in the recipient's peripheral blood, bone marrow or other tissue. Shalini Pereira, Ph.D.
Views: 431 UWTV
Dr. Erba Describes Proper Diagnostic Testing of CML
Harry Erba, MD, PhD, associate professor, Department of Internal Medicine, University of Michigan Health System, discusses the full histopathological examination that should be performed when a patient is diagnosed with chronic myeloid leukemia (CML). If the routine complete blood count shows leukocytosis, a shift in maturity, basophilia, and splenomegaly, 98 times out of 100 the patients will have CML, possibly in the chronic phase. This holds true whether constitutional symptoms are present or not. There are ways to make a non-invasive diagnosis for CML using fluorescence in situ hybridization (FISH) or polymerase chain reaction (PCR) on the peripheral blood to check for a BCR-ABL gene rearrangement. However, Erba does not recommend using this method, stating that CML requires a more thorough baseline analysis. Bone marrow biopsy is critical at the time of diagnosis to provide morphologic evidence of the phase. The biopsy can show fibrosis or the clustering of mature cells indicating whether the cancer is in chronic, accelerated, or blast phase. Knowing the phase of the disease will help choose an appropriate therapy. A cytogenetic analysis of the chromosomes at metaphase, the most condensed and easiest to distinguish phase in the cell cycle, may demonstrate clonal evolution or other changes that could upgrade the patient to a higher phase. The analysis may also show that some patients are not Philadelphia chromosome positive at metaphase, making it less likely to mistake a negative test later as a complete cytogenetic remission. Additionally Erba recommends the completion of a FISH analysis at the time of diagnosis. Approximately 10% of patients have a variant FISH pattern, which is important to know early on. The final test that Erba recommends is a reverse transcription (RT)-PCR. The results of this test can be used as a baseline for response and to verify that the primers used in most commercial laboratories for detecting the BCR-ABL transcription are applicable. Some patients demonstrate variant break points, which makes it easier to arrive at the wrong conclusions if the RT-PCR test is not completed.
Views: 1004 OncLiveTV
CTMM SME SkylineDiagnostics
Presentation of Skyline Diagnostics at the SME Exhibition Fair during the 4th CTMM Annual Meeting on Sept 13, 2012, Jaarbeurs Utrecht, NL. The Center for Translational Molecular Medicine (CTMM: www.ctmm.nl); a Dutch public-private partnership enables earlier and more precise diagnosis of disease and the design of highly personalized therapies. At the 2012 CTMM Annual Meeting, representatives from all 22 CTMM projects were able to demonstrate the real success that was being achieved in moving the results of fundamental research ever closer to patient care. Skyline Diagnostics, partner in the CTMM BioCHIP project, develops and markets microarray-based diagnostic tests using molecular signatures for personalized medicine in oncology. With its infrastructure, expertise and access to large collections of well-defined clinical samples, Skyline is an excellent partner for discovery, development and commercialization of new diagnostic assays and devices. In this video, Skyline Diagnostics is demonstrating its AMLProfiler. The AMLProfiler is a comprehensive diagnostic test for Acute Myeloid Leukemia (AML), a device that simultaneously analyses several different diagnostic and prognostic markers on the same microarray. Combined with its underlying IT infrastructure, the AMLProfiler can lead to standardized cost-effective laboratory workflows that enable physicians to make precise diagnoses of AML subtype within four days. This helps clinicians to choose the most appropriate available treatment for each patient and perhaps more importantly, create valuable time to search a donor for a matching bone marrow transplant, resulting in better patient outcomes. The AMLProfiler was launched in Europe as a diagnostic service in December 2009 and is offered as an in-vitro diagnostic (IVD) test. In the USA, CTMM partner Skyline was the first company to apply for FDA Pre-Market Approval (PMA) for a diagnostic microarray.
Phases of Chronic Myeloid Leukemia
Periods of Chronic Myeloid Leukemia. What is unending myeloid leukemia?. Interminable myeloid leukemia, or CML, is one of four sorts of malignancy that starts in the bone marrow. It begins in the blood-framing cells and can inevitably spread all through the body by means of the blood. Since CML is a ceaseless type of tumor, it goes ahead bit by bit and goes on for a considerable length of time. This varies from intense kinds of leukemia, which start abruptly and are quickly dangerous. CML is caused by a hereditary transformation that outcomes in platelets creating excessively of the tyrosine kinase protein. This protein causes an overproduction of sick platelets, and enables them to live too long. In the long run, these ailing cells rule out solid platelets. Periods of CML: There are three periods of CML: endless, quickened, and blastic. The stage is controlled by the movement of the malady and is estimated by the quantity of impact cells. Impact cells are unusual white platelets that don't develop appropriately and swarm out solid cells. Endless: The primary period of CML is the perpetual stage. The vast majority are determined to have CML amid this stage. Amid the endless stage, disease cells develop and increase gradually. This stage can last inconclusively and indications are exceptionally mellow or not present by any means. Those with CML may not know they have the illness for quite a long while. A finding for the most part comes about because of blood tests given for an inconsequential reason. Quickened: A moderately modest number of individuals will advance from the ceaseless stage to the quickened stage. In the quickened stage, the impact cells develop and duplicate all the more rapidly, and they appear to quit reacting to treatment. Individuals in this stage may see manifestations like weariness, caused by a lower number of red platelets, and stomach torment because of a broadened spleen. Different highlights of this stage incorporate a low platelet tally and strangely high or low quantities of white platelets. Blastic: On the off chance that left untreated, those with CML can advance to the third stage. In the blastic stage, the impact cells have achieved a basic level. These levels are here and there equivalent to those found in individuals with intense leukemia. This surge of impact cells prompts perilously low levels of red platelets and platelets. Indications are more extreme, and can include: *rash. *bruising and draining effortlessly. *bone and joint agony. *stomach torment. *more articulated weariness. *difficulty relaxing. *increased danger of contamination. *night sweats. *weight misfortune. Amid the blastic stage, CML is hard to treat and is thought to be dangerous. Treatment alternatives: At the point when analyzed in the ceaseless stage, CML is generally first treated with drugs called tyrosine kinase inhibitors, or TKIs. TKIs are meds that objective the protein created by the harmful cells. This sort of treatment is regularly enough to place CML into abatement. At times, oral chemotherapy is likewise used to kill off some white platelets, if white cell levels have turned out to be too high. Despite the fact that these medications can't totally cure CML, individuals typically react great and stay going away for a long time. More forceful treatment choices must be considered for those in the quickened stage. Undifferentiated cell transplants or bone marrow transplants are the following course of treatment, and can bring about a genuine cure. Transplants are additionally prescribed for the individuals who have advanced to the blastic stage. Treatment is considerably more troublesome amid this stage in light of the fact that there are less solid cells. Remaining solid: It's imperative to take after your specialist's prescribed course of treatment when you're determined to have CML. Holding up until the point that you have repulsive side effects or until the point when you have come to the quickened or blastic stage can make your CML considerably harder to treat. Myeloid Leukemia,Chronic Myeloid Leukemia,what is leukemia,Phases of Chronic Myeloid Leukemia,What is unending myeloid leukemia?,what is chronic myeloid leukemia,acute myeloid leukemia,leukemia,chronic myeloid,myeloproliferative disorders,chronic myeloid leukemia treatment,chronic myeloid leukemia causes,symptoms of leukemia,leukemia symptoms,acute leukemia,chronic myeloid leukemia symptoms,leukemia rash,signs of leukemia,what causes leukemia,cancer All Photos Licensed Under CC Source : www.pexels.com www.pixabay.com www.commons.wikimedia.org
Views: 2846 5 Minute Remedies
Molecular Video Lecture 01:  Variants, Nomenclature, Nucleic Acid
Topics: * Germline vs somatic testing 1:14 * Analytical sensitivity in germline testing 4:40 * Analytical sensitivity in somatic testing 6:34 * Sanger sequencing limit of detection 10:20 * Important of preanalytic assessment of tumor cellularity 10:39 * Different types of variants/mutations 12:57 * Single nucleotide variants or polymorphisms/point mutations 13:39 * SNV/point mutation consequences 14:46 * Nonsense variants 15:09 * Missense variants 15:33 * Synonymous variants 15:54 * Nonsynonymous variants 16:26 * Indel variants 17:00 * Frameshift variants 17:20 * Copy number variants 18:14 * Rearrangements 19:25 * Translocations, balanced vs unbalanced 20:45 * Inversions, peri- vs para-centric 21:08 * Epigenetic changes 22:38 * Methylation 22:50 * Histone modification 23:25 * Nomenclature 24:27 * HGNC 25:30 * HGVS 26:39 * HGVS for SNVs (DNA) 27:03 * HGVS for splice/intronic variant (DNA) 32:19 * HGVS for indels (DNA) 34:14 * HGVS for SNVs (protein) 36:37 * Nucleic acid extraction 39:10 * Intracellular biochemical substrates 39:25 * Preanalytical circling of tumor on H&E 42:06 * Coring vs. scraping 43:00 * Blood/bone marrow specimens 46:32 * Formalin fixed paraffin embedded (FFPE) tissue 46:52 * Cell lysis 47:51 * Nucleic acid isolation 51:50 * Isolation by silica membrane column 52:10 * Phenol/cholorform extraction/alcohol precipitation 53:35 * SPRI bead based isolation 56:10 * Ultracentrifugation 58:06
Views: 1232 long le
FFPE FISH Protocol
Our pretreatment kit is capable of preparing slides for CISH and/or FISH analysis on formalin-fixed, paraffin-embedded (FFPE) tissue. Watch our video showing step-by-step instructions on how to use the kit with our extensive Aquarius® Pathology FISH probe range.
Stem Cell Therapy for Leukemia
Full Lesson Here: http://ed.ted.com/on/g7P19NfN Dubay points out that stem cell therapy dates back to the 1950's and highlights some landmark discoveries in the treatment of leukemia from HLA matching to the use of cord blood.
Views: 1637 Dan Dubay
HANABI HARVESTER PII - One Protocol_Different samples sources - ADS Biotec
Blood and Bone Marrow samples in one run.
Views: 227 ADS Biotec Omaha
AbOriginal Black Man Adam's Rib XY Bone & Eve XX Chromosomes! Ask RasTafari Rabbi YadOn
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How Does A Chimera Happen?
Second, chimeras can arise when developing fraternal twins share a blood supply. Googleusercontent search. Human chimeras were first discovered with the advent of blood typing when it was found that some people had more than one type. This condition occurs through the fertilisation of two separate ova by sperm, followed aggregation at blastocyst or zygote stages. In human beings, a chimera is person who has two totally different sets of dna inside their body 7 nov 2017 people that have are called chimeras. Till date, 35 chimera people in the us have been identifiedhow does chimerism occur embryo? When was first discovered? How is chemerism detected? What are features of. It can also happen after a bone marrow transplant, and (in smaller scale) during normal pregnancy 8 jun 2004 fraternal twins do not have the same dna, so mixture of two embryos will give chimera. Nov 2017 people that have two different sets of dna are called human chimeras. Previous cases of chimeras revealed people with cells that contained dna different from their own. Human chimerism hit the condition is relatively common in some animals but it may happen more often than we realise humans, and go unnoticed. For example, in 2002, news outlets reported the story of a woman named karen keegan, who needed kidney transplant and underwent genetic testing along with her family, to see if family member could donate one humans, condition can occur naturally, though it typically ends embryonic death. First human pig 'chimera' created in milestone study the embryogenesis of chimeras, twins and anterior midline asymmetries chimeras they do exist what is chimerism? . Think of a chimera as twins that melded in medicine, person composed two genetically distinct types cells. Mothers retain some cells of their children, and a recent danish study found that women who gave birth to boys retained with y chromosomes 5 jan 2017 the aim is create chimera animals can grow organs order. Put another way, the chimera is formed 5 aug 2016 one way chimeras can happen naturally in humans that a fetus absorb its twin. Most of them proved to be 'blood chimeras' non identical twins who shared a blood supply in the 26 jan 2017 garry said that rapid progress chimera research had prompted range troubling questions, including whether progeny would look more human or pig, what happen if thought and it was possible for cells cannibalise pig embryo, definition churchill's medical dictionary (1989) mentions somatic mutation as source chimerism, but goes on say 'it occurs humans most commonly when dizygous mixes utero. The definition in online medical dictionary (2004) does not mention mixing of bloods, but offers some forms genetic and scientific research do appear controversial at outset, like researchers wanting to create human animal chimeras. What is a human chimera and how does it happen? Insider thisisinsider what happen 2017 11 class "imx0m" url? Q webcache. Chimeras understanding genetics the. 28 oct 2015 suc
Views: 21 tell sparky
What is FEMALE SPERM? What does FEMALE SPERM mean? FEMALE SPERM meaning & explanation
What is FEMALE SPERM? What does FEMALE SPERM mean? FEMALE SPERM meaning - FEMALE SPERM definition - FEMALE SPERM explanation. SUBSCRIBE to our Google Earth flights channel - https://www.youtube.com/channel/UC6UuCPh7GrXznZi0Hz2YQnQ Source: Wikipedia.org article, adapted under https://creativecommons.org/licenses/by-sa/3.0/ license. Female sperm can be either: 1. A sperm that contains an X chromosome, produced in the usual way by a male, referring to the occurrence of such a sperm fertilizing an egg and giving birth to a female. 2. Sperm containing genetic material from a female. We'll focus on 2nd definition in this video. Since the late 1980s, scientists have explored how to produce sperm where all of the chromosomes come from a female donor. In the late 1990s, this concept became a partial reality when scientists in Japan developed chicken female sperm by injecting bone marrow stem cells from a female chicken into a rooster's testicles. This technique proved to fall below expectations, however, and has not yet been successfully adapted for use on humans. Creating female sperm was first raised as a possibility in a patent filed in 1991 by injecting a woman's cells into a man's testicles, though the patent focused mostly on injecting altered male cells into a man's testes (to correct genetic diseases). In 1997, Japanese scientists partially confirmed such techniques by creating chicken female sperm in a similar manner. "However, the ratio of produced W chromosome-bearing (W-bearing) spermatozoa fell substantially below expectations. It is therefore concluded that most of the W-bearing PGC could not differentiate into spermatozoa because of restricted spermatogenesis." These simple transplantation methods follow from earlier observations by developmental biologists that germ stem cells are autonomous in the sense that they can begin the processes to become both sperm and eggs. One potential roadblock to injecting a woman's cells into a man's testicles is that the man's immune system might attack and destroy the woman's cells. In usual circumstances, when foreign cells (such as cells or organs from other people, or infectious bacteria) are put into a human body, its immune system will reject such cells or organs. However, a special property of a man's testicles is that they are immune-privileged, that is, a man's immune system will not attack foreign cells (such as a woman's cells) injected into the sperm-producing part of the testicles. Thus, a woman's cells will remain in the man's testicles long enough to be converted into sperm. However, there are more serious roadblocks. Biologists have well established that male sperm production relies on certain genes on the Y chromosome, which, when missing or defective, lead to such men producing little to no sperm in their testicles. An analogy, then, is that a cell from a woman has complete Y chromosome deficiency. While many genes on the Y chromosome have backups (homologues) on other chromosomes, a few genes such as RBMY on the Y chromosome do not have such backups, and their effects must be compensated for to convert cells from a woman into sperm. In 2007, a patent application was filed on methods for creating human female sperm using artificial or natural Y chromosomes and testicular transplantation. Key to successful creation of female sperm (and male eggs) will be inducing male epigenetic markings for female cells that initially have female markings, with techniques for doing so disclosed in the patent application. Scientists from the University of Newcastle upon Tyne led by biologist Karim Nayernia discovered a method of creating partly developed sperm cells, otherwise known as "spermatogonial" stem cells, from the bone marrow of male volunteers, entirely in-vitro (outside the human body), and is seeking funding to see whether such techniques can be used to make female sperm. If created, a "female sperm" cell could fertilize an egg cell, a procedure that, among other potential applications, might enable female same-sex couples to produce a child that would be the biological offspring of its two mothers. It is also claimed that production of female sperm may stimulate a female to be both the mother and father (similar to asexual reproduction) of an offspring produced by her own sperm. Many queries, both ethical and moral, arise over these arguments.
Views: 415 The Audiopedia
4) Dr.Gihan [Cytogenetics] 16/11/2013
Cytogenetics : - The cell cycle - Cell division - Human chromosome
The issue of relapse in Philadelphia chromosome-positive ALL patients and how to combat this
Philippe Rousselot, MD, PhD from Versailles Hospital and University, Versailles, France discusses the current medical need of Philadelphia chromosome-positive (Ph+) acute lymphoblastic leukemia (ALL) patients. After five years of follow up, most studies have shown a relapse rate of around 60%, despite the combination of tyrosine kinase inhibitors (TKI’s, mainly imatinib and chemotherapy), with various dosage levels, as well as the aim of allogeneic bone marrow transplantation. Prof. Rousselot, goes on to discuss a trial (NCT00391989) testing dasatinib as first-line treatment in elderly ALL patients. Very few of the participants had been allografted and of those that relapsed, analysis showed an association with the T315I mutation. The T315I mutation is able to be detected before relapse and at diagnosis, making it possible to better characterize these patients. Prof. Rousselot ends the interview by mentioning a new approach to combating relapse, monoclonal antibodies. These were evaluated in relapsed/refractory patients, and are now on the way to being introduced in the frontline setting. He believes the possibility to combine a monoclonal antibody with a TKI will be available within one year. Recorded at the European Hematology Association (EHA) 2016 Annual Congress in Copenhagen, Denmark.
What Is The Definition Of AML Medical Dictionary Free Online
what is the definition of AML: Acute myeloid leukemia (also known as acute myelogenous leukemia), a quickly progressive malignant disease in which there are too many immature blood-forming cells in the blood and bone marrow, the cells being specifically those destined to give rise to the granulocytes or monocytes, both types of white blood cells that fight infections. In AML, these blasts do not mature and so become too numerous. AML can occur in adults or children. Acute myeloid leukemia is also known as acute myelogenous leukemia or acute nonlymphocytic leukemia (ANLL). The early signs of AML may be similar to the flu or other common diseases with fever, weakness and fatigue, loss of weight and appetite, and aches and pains in the bones or joints. Other signs of AML may include tiny red spots in the skin, easy bruising and bleeding, frequent minor infections, and poor healing of minor cuts. First, blood tests are done to count the number of each of the different kinds of blood cells and see whether they are within normal ranges. In AML, the red blood cell levels may be low, causing anemia; platelet levels may be low, causing bleeding and bruising; and the white blood cell levels may be low, leading to infections. A bone marrow biopsy or a bone marrow aspiration may be done if the results of the blood tests are abnormal. During a bone marrow biopsy, a hollow needle is inserted into the hip bone to remove a small amount of the marrow and bone for examination under a microscope. In a bone marrow aspirate, a small sample of liquid bone marrow is withdrawn through a syringe. A lumbar puncture, or spinal tap, may be done to see if the disease has spread into the cerebrospinal fluid, which surrounds the central nervous system (CNS) -- the brain and spinal cord. Other key diagnostic tests may include flow cytometry (in which cells are passed through a laser beam for analysis), immunohistochemistry (using antibodies to distinguish between types of cancer cells), cytogenetics (to determine chromosome changes in cells), and molecular genetic studies (DNA and RNA tests of the cancer cells). The primary treatment of AML is chemotherapy. Radiation therapy is less common; it may be used in certain cases. Bone marrow transplantation is under study in clinical trials and is coming into increasing use. There are two phases of treatment for AML. The first phase is called induction therapy. The purpose of induction therapy is to kill as many of the leukemia cells as possible and induce a remission, a state in which there is no visible evidence of disease and blood counts are normal. Patients may receive a combination of drugs during this phase including daunorubicin, idarubicin, or mitoxantrone plus cytarabine and thioguanine. Once in remission with no signs of leukemia, patients enter a second phase of treatment. The second phase of treatment is called post-remission therapy (or continuation therapy). It is designed to kill any remaining leukemic cells. In post-remission therapy, patients may receive high doses of chemotherapy, designed to eliminate any remaining leukemic cells. Treatment may include a combination of cytarabine, daunorubicin, idarubicin, etoposide, cyclophosphamide, mitoxantrone, or cytarabine. There are a number of different subtypes of AML. AML is classified using a system called the French American British (FAB) system. In the this system, the subtypes of AML are grouped according to the particular cell line in which the disease developed. There are eight distinct types of AML, designated M0 through M7. Types M2 (myeloblastic leukemia with maturation) and M4 (myelomonocytic leukemia) each account for 25% of AML; M1 (myeloblastic leukemia, with few or no mature cells) accounts for 15%; M3 (promyelocytic leukemia) and M5 (monocytic leukemia) each account for 10% of cases; the other subtypes are rarely seen. AML is also classified according to the chromosomal abnormalities in the malignant cells. medical terminology, medical dictionary, medical dictionary free download, medical terminology made easy, medical terminology song
Breaking Leukemia's Limits of Detection with Droplet Digital™ PCR
For more info, visit http://www.bio-rad.com/droplet. Prof. Alec Morley, a pioneer in digital PCR, describes his use of Droplet Digital PCR (ddPCR™) technology to advance the diagnosis and treatment of leukemia. Morley and his colleagues at Flinders University and Medical Center in Adelaide, South Australia previously developed a digital PCR method for the early detection of residual acute lymphoblastic leukemia (ALL) using a genetic marker instead of traditional microscopy of bone marrow cells, enabling more accurate prediction of relapse risk for ALL patients following chemotherapy. Monoquant, a company founded by Morley to commercialize the research in his lab, has now chosen Bio-Rad's ddPCR System to develop a new clinical test for chronic myeloid leukemia (CML). The unparalleled sensitivity of ddPCR technology promises great improvement over conventional qPCR tests and also eliminates the variability in amplification efficiency that results from using patient-specific PCR primers, removing a potential roadblock to FDA approval. The QX200™ Droplet Digital PCR System, Bio-Rad's second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications.
Views: 1453 Bio-Rad Laboratories
An Integrative Translational Study of Acute Myeloid Leukemia
Leukemia is a special topic of study. The discovery of Philadelphia chromosome in chronic myeloid leukemia (CML) in 1960s established a concept that cancers are genetic diseases. The introduction of imatinib for treatment of CML around year 2000 opened the era of target therapy. Through the contributions of scientists, our understanding of leukemia as well as the policies of treatment has dramatically changed. Acute myeloid leukemia (AML) is an aggressive disease with the highest annual incidence among leukemia in Taiwan. The prognosis has significantly improved, largely because of personalized treatment through fine stratification of the patients’ risk. We have been devoted to the translational study in AML in the past 10 years, thanks to the relative easy collection of tumor cells. The theme of our study is starting from sample archival; we collected decades of serial patients’ samples for genetic studies, including chromosomal analysis and mutation identification, by which we obtained a general clinical and genetic demographic picture and identified genetic markers for prognostication. We then proceeded to global mRNA and microRNA expression profiling, whereby we were able to identify expression signatures of prognostic significance and novel genetic markers critical to patients’ response to treatment. These efforts have further contributed to better selection of optimal treatment regimens for individual patients. In addition, the novel markers led us to explore their roles in prognosis and pathogenesis. We have also established a platform of animal modelling of AML, through which we could test the biological functions via in vivo experiments. In summary, our research platform is an integrative, bedside to bench approach, in which we get insights and generate hypotheses from clinical information and proceed to a mechanistic exploration through in vitro and in vivo animal studies. 主講人:周文堅 教授 (臺大醫院檢驗醫學部及內科部主治醫師) 拍攝日期:2015/10/12
Views: 287 臺大演講網
Mutation Analysis in Chronic Myeloid Leukemia (CML)
The panelists, Jorge Cortes, MD; Kevin Kelly, MD, PhD; Harry Erba, MD, PhD; Javier Pinilla-Ibarz, MD, PhD; and David Snyder, MD, FACP, explain technological advances in mutation analysis for chronic myeloid leukemia (CML).
Views: 1003 OncLiveTV
How Many Chromosomes Are In A Chimpanzee?
Male human chromosome more similar to gorillas than ape differences. Please print many of the chimpanzee chromosomes have bands heterochromatin (illustrated as yellow) either 26 feb 2014 article received so much attention that a follow up seemed in order; After all, humans and chimpanzees, aligned for on chimp chromosome #13 there 3 human does not. ' 1 21 jun 2006 human and chimpanzee chromosome differ in terms of their number and also in regard to chromosomal banding patterns humans have 23 pairs of chromosomes while great apes have 24 pairs. Stackexchange evolutionarily speaking why do humans have 46 chromosomes url? Q webcache. Chromosome 2 what separates chimps from humans? Youtube. Humans have 23 pairs of chromosomes and other great apes 24 16 nov 2004 since the y chromosome pretty much only carries instructions for making males, humans chimps probably about same number 14 jan 2010 so 98 percent. Mar 2016 surprisingly, we found that in many ways the gorilla y chromosome is more be aligned to human than chimpanzee humans have a characteristic diploid number of 2n 46 whereas other great apes (orangutans, gorillas, and chimps) are all 48Evolutionarily speaking, why do chromosomesi realize chromosomes chimps 48, but. Monkeys, chimpanzees, and apes have 24 pairs (twenty four pairs), for a total of 48 human chimpanzee chromosomes are very similar. Unbelievable y chromosome differences between humans and new research undermines human chimp similarity chimpanzee chromosomes similarities differencesthe mystery of the missing (with a special guest are remarkably divergent chimps. Chimpanzee & human chromosomes living links. 30 aug 2012 evolutionarily speaking, why do humans have 46 chromosomes. Googleusercontent search. Evolutionarily speaking, why do humans have 46 chromosomesi realize that chromosomes and chimps 48, but we. Of apes and men chromosome 2 in humans the chimpanzee. In the y chromosome p arms are homologous and much of q arm is 16 dec 2010 there many candidate regions in genome, but new data on chromosomes chimpanzees may have shaken them out apes men 2 humans chimpanzee beyond scope this article, though could be said response to claim particular, it explains that one fewer pair their proposed great which include chimpanzees, gorillas, fact, mutations thought neutral, some even give rise 1 2013. Evolution library human chromosome 2 pbs. Percent of your dna with this sex obsessed ape comparison human and chimpanzee chromosomes chimp y chromosome creation. The primary difference is that humans have one fewer pair of chromosomes than do other great apes. In humans, each cell normally contains 23 pairs of chromosomes, for a total 46. Evolutionarily speaking, why do humans have 46 chromosomes biology. Humans carry one fewer chromosome than chimpanzees, bonobos, gorillas and 19 jul 2012 the chimp genome team were able to match human two even if it unique, there's much less of you would expect 13 jan 2010 y began evolve from an autosome chimpan
Views: 81 Sea of Question
From Stem Cells To Cancer Cells
Fernando Camargo discusses his research on how cancer arises, what can be done to stop it, and whether cancer is related to stem cells. The human stem cells found in our bone marrow have the amazing ability to develop into different types of cells such as blood or immune system cells. This happens in all of us everyday, but we know little about how it happens. Camargo's research centers on figuring out how stem cells travel down their path to become a new bone or blood cell. Recently, new theories suggest that stem cells have some of the same characteristics as cancer cells. Figuring out how stem cells replicate themselves, divide into specialized cells, or even give rise to a cancer would provide answers for research scientists and maybe treatments for patients.
Views: 346 WGBHForum
Progenesis IVF Center Video 1
Progenesis Fertility Center, Test tube baby, test tube baby centre in Nasik, test tube baby clinic in india,infertility clinic in nasik Maharashtra, Dr. Narhari S. Malagaonkar (Aher), Dr. Sonali N. Malagaonkar , infertility centre in nashik, Progenesis Fertility Center in Maharashtra, infertility centre in india,infertility clinic in india,IVF in Maharashtra, IVF nashik, IVF Centre in Nasik India, Male Infertility, Female Infertility Clinics, Obstetrics Treatments, Progenesis Fertility Center in india, Gynecology Treatments, Pregnancy Hospitals, High Risk Pregnancy, Artificial Insemination, IUI, ICSI, Egg, Egg Donation, Embryo, Embryo Donation, Blastocyst, Semen, Semen Banking, Semen Production, Post-Coital Test, Anti Body Test, Diagnostic Semen Wash, Morphology, Semen Analysis, Biopsy, Basal Body Temperature Chart, BBT, Follicular Study, Progesterone Level, Endocrine Studies, Antisperm Antibody Testing, Endometrial, Biopsy, Hysterosalpingogram, HSG, Diagnostic Hysteroscopy, Diagnostic Laparoscopy, Nashik test tube baby centre, nasik test tube clinic, nasik infertility clinic, infertility mp, Fertility treatments, fertilization, ivf treatment, intracytoplasmic sperm injection, test tube baby, mesa test tube baby, microscopic epididymal sperm aspiration, ovarian drilling, egg donation, embryos freezing, sperm freezing, endoscopy, molecular microbiology, prenatal diagnosis, diagnose anemia, chromosomal analysis, fluorescent in situ hybridization, dna diagnosis, ovarian drilling for polycystic ovarian syndrome, egg donation for baby, female fertility by test tube baby, female fertility centre, laparoscopy centre, hysteroscopy centre, azoospermia centre, genetic centre, nashik, india, Test tube bay center, test tube baby india, test tube clinic, egg donation, egg donors
Views: 1081 ivf center
Okamoto placenta-derived cardiomyocytes
This Confocal image was published in Exp Cell Res. 313:2550-62, 2007. The title is 'Working' cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells." by Okamoto K, Miyoshi S, Toyoda M, Hida N, Ikegami Y, Makino H, Nishiyama N, Tsuji H, Cui CH, Segawa K, Uyama T, Kami D, Miyado K, Asada H, Matsumoto K, Saito H, Yoshimura Y, Ogawa S, Aeba R, Yozu R, Umezawa A. The experiment was performed at Department of Reproductive Biology and Pathology, National Research Institute for Child Health and Development, Tokyo, Japan. Abstract of this study is as below: The clinical application of cell transplantation for severe heart failure is a promising strategy to improve impaired cardiac function. Recently, an array of cell types, including bone marrow cells, endothelial progenitors, mesenchymal stem cells, resident cardiac stem cells, and embryonic stem cells, have become important candidates for cell sources for cardiac repair. In the present study, we focused on the placenta as a cell source. Cells from the chorionic plate in the fetal portion of the human placenta were obtained after delivery by the primary culture method, and the cells generated in this study had the Y sex chromosome, indicating that the cells were derived from the fetus. The cells potentially expressed 'working' cardiomyocyte-specific genes such as cardiac myosin heavy chain 7beta, atrial myosin light chain, cardiac alpha-actin by gene chip analysis, and Csx/Nkx2.5, GATA4 by RT-PCR, cardiac troponin-I and connexin 43 by immunohistochemistry. These cells were able to differentiate into cardiomyocytes. Cardiac troponin-I and connexin 43 displayed a discontinuous pattern of localization at intercellular contact sites after cardiomyogenic differentiation, suggesting that the chorionic mesoderm contained a large number of cells with cardiomyogenic potential. The cells began spontaneously beating 3 days after co-cultivation with murine fetal cardiomyocytes and the frequency of beating cells reached a maximum on day 10. The contraction of the cardiomyocytes was rhythmical and synchronous, suggesting the presence of electrical communication between the cells. Placenta-derived human fetal cells may be useful for patients who cannot supply bone marrow cells but want to receive stem cell-based cardiac therapy.
Views: 540 Akihiro Umezawa
#TomorrowsDiscoveries: DNA Rearrangements That Cause Childhood Cancer - Dr. Stephen Desiderio
#TomorrowsDiscoveries: To battle invaders, our immune system deploys antibodies, made by shuffling bits of DNA. But if the DNA and developing immune cells are misshuffled, they can cause childhood cancer. Dr. Stephen Desiderio and his team study these DNA rearrangements, and they’ve discovered ways the immune system protects us from damage to our genes, gaining insight into the causes of leukemia. Learn more about Dr. Desiderio’s work: http://ow.ly/PrLzy
Living Well With Myeloma: Making Sense of Tests and Results
Join the IMF’s Medical Editor Debbie Birns and Dr. Rafat Abonour for a “Living Well with Myeloma” webinar on Making Sense of Tests and Results. Debbie will outline the various tests that are used to diagnose myeloma, monitor response to treatment, and detect side effects. Please subscribe to our channel! Subscribe to International Myeloma Foundation: http://bit.ly/XlUtPE Visit our website at: https://www.myeloma.org Find us online: Facebook: http://facebook.com/myeloma Google+: http://gplus.to/imfmyeloma IMF on twitter: @IMFMyeloma (http://twitter.com/imfmyeloma) Dr. Durie on twitter: @BrianDurieMD (http://twitter.com/brianduriemd) Support the IMF! http://bit.ly/WskQHC Category Nonprofits & Activism License Standard YouTube License